In Dec 2025, we noticed that a few whole genome alignments have been missing certain local alignments in the alignment chains. This can affect the TOGA runs and increase the number of missing genes. We therefore recomputed the chains and re-ran TOGA2 for the following species: for reference assembly hg38 the following query assemblies were affected: HLapoSpe1 HLcarSowe1A HLcasYok2B HLcheLug1 HLcheReg1 HLchiIsr1 HLcolGue1 HLcorRaf1 HLcunPaca1 HLdesRot12A HLdesRot12B HLescRob3 HLgeoBur1 HLglaVol1 HLhapAlao1 HLmanSph3 HLmicOre1 HLmunCri2 HLmyoCal2 HLnycThe1B HLodoHem2 HLpagLar1 HLpenLuca1A HLpitPit2 HLpleAur1 HLpusHisBot1 HLsaiSci1 HLzalCali2 calJac4 for reference assembly mm10 the following query assemblies were affected: HLartLitu2B HLateCha1 HLcasYok1B HLcerAlb1 HLeulCoro1 HLgalSen1 HLictTrid4A HLictTrid4B HLlepYar1 HLmerPsa1 HLmesBid2B HLmirCoq2 HLmyoEma1B HLmyoLuc2 HLmyoMys2B HLmyoNat1A HLneoFus1 HLotoCra2 HLpagLar1 HLpipAbr1 HLpipJav1 HLplaHell1B HLpseFum1 HLpteAle1A HLrhiPum1A HLrhyPet1A HLscoKuh1 HLtapIndi3A HLtapIndi3B HLtokOsi1 HLtupChi2 HLtylFul1 HLuroPar2A HLursAme3 HLxanPyg1 for reference assembly HLbosTau10 the following query assemblies were affected: HLariCup2A HLartInt1B HLcapSib1 HLcarPer3 HLcatWag1 HLdicSum1 HLdicSumHar2 HLequAsin4 HLeryPat3 HLeubGla2 HLgarKeen1A HLgloCom1A HLhipAmp3 HLhooLeu1 HLictTrid4A HLlepYer2A HLlopEvot1A HLmacFas7 HLmegLyr2 HLmegNova3 HLmesSte1 HLmicCal1A HLmicGri1 HLmicPen1A HLminAus1 HLminNat2B HLmopPli1 HLmusNiv1 HLmyoBoc1 HLmyoEma1A HLneoNeb2 HLneoShe1 HLokaJoh2 HLotaByr1 HLphyCat2 HLpleAur1 HLpteGig1 HLpteParMex3 HLpteVam3 HLratRat7 HLrhiHip1A HLrhiPum1B HLrhoPar1 HLscoTegu1 HLspiGra1 HLtenEcau2A HLtriPer1A HLuroTal1 HLvarVar1 cavApe1 for reference assembly HLeleMaxInd3A the following query assemblies were affected: HLailMel2 HLaleOec1 HLammLer1 HLartLit1A HLbabCel1 HLbalBor1 HLbisBon1 HLcapHir2 HLcerEla2 HLchiIsr1 HLchlSab3 HLdipSag1 HLeliQue1A HLequAsin4 HLeulCol1 HLfelCha1 HLhelMal1 HLhelPar1 HLhipArm1 HLhipArm2 HLhipNigNig2 HLiaxIox1 HLictTrid4B HLlopAte1 HLlutLut1 HLmacSil1 HLmacWat1B HLmesSte1 HLmicCal1B HLmolAlv2B HLmunMun1 HLmunVag1 HLmusErm1 HLmusPutFur2 HLmyoMys2A HLnanDamRuf3 HLneoVis2 HLotaByr1 HLoviArie7 HLoviNivLyd1 HLperCri1 HLphaAfr1 HLpleCal1 HLpusHisBot1 HLrhiAff4 HLrhyNas2A HLsagImp1 HLsapApe3 HLspiGra1 HLsusScro12 HLtamTet2 HLtapInd1 HLtenEcau2A HLtriNic1B HLuroPar1 HLuroTal1